0.2 M Sodium acetate Buffer ; pH 4.0
Prepare a 0.2M Sodium Acetate Solution. Weigh 27.2 gm of Sodium Acetate Trihydrate into a one liter volumetric flask. Add 800 mL of Deionized Water. Mix and dissolve. Bring the pH down to 4.0 with Glacial Acetic Acid. Finally adjust the volume to one liter with Deionized Water.
0.2 M Sodium acetate Buffer with Column Activator ; pH 4.0
Prepare a 0.2M Sodium Acetate Solution. Weigh 27.2 gm of Sodium Acetate Trihydrate into a one liter volumetric flask. Add 800 mL of Deionized Water. Mix and dissolve. Bring the pH down to 4.0 with Glacial Acetic Acid. Add 70 mL of n-Propanol. Finally adjust the volume to one liter with Deionized Water and mix.
ATP Releasing Reagent
0.2 M Sodium acetate Buffer ; pH 5.0
Prepare a 0.2M Sodium Acetate Solution. Weigh 27.2 gm of Sodium Acetate Trihydrate into a one liter volumetric flask. Add 800 mL of Deionized Water. Mix and dissolve. Bring the pH down to 5.0 with Glacial Acetic Acid. Finally adjust the volume to one liter with Deionized Water.